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phospho src tyr 416  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phospho src tyr 416
    Phospho Src Tyr 416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+src+tyr+416/pmc13053057-177-68-71
    Average 86 stars, based on 1 article reviews
    phospho src tyr 416 - by Bioz Stars, 2026-10
    86/100 stars

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    Membrane:

    Article Title: An Extracellular Matrix–Producing Subset of Cancer-Associated Fibroblasts Drives Chemoresistance in Breast Cancer via SRC Activation and G0S2 Upregulation
    Article Snippet: Protein migration was performed for 1.5 hours at 150 V in 1× NuPAGE MES SDS Running Buffer (Invitrogen, #NP002). .. Proteins were then transferred to a 0.45-μm nitrocellulose membrane (GE HealthCare, #10600002) and incubated overnight at 4°C with the appropriate primary antibodies: 1:50,000 β-actin (Proteintech, t#66009-1-Ig, RRID: AB_2687938), 1:1,000 G0S2 (Proteintech, cat. #12091-1-AP, RRID: AB_2877824), and 1:2,000 SRC (Cell Signaling Technology, #2110, RRID:AB_10691385) in Tris-buffered saline-Tween 20 (TBST; TBS tablets: EUROMEDEX, #EU1-7500-100; Tween 20: Sigma-Aldrich, #P2287) supplemented with 5% nonfat dry milk (Cell Signaling Technology, #999S) or 1:2,000 phospho-SRC Tyr 416 (Cell Signaling Technology, cat. #6943, RRID: AB_10013641) in TBST supplemented with 5% BSA (EUROMEDEX, # 04-100-812-C). .. On the next day, membranes were washed three times with TBST and incubated with the appropriate peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, #115-035-003).

    Incubation:

    Article Title: An Extracellular Matrix–Producing Subset of Cancer-Associated Fibroblasts Drives Chemoresistance in Breast Cancer via SRC Activation and G0S2 Upregulation
    Article Snippet: Protein migration was performed for 1.5 hours at 150 V in 1× NuPAGE MES SDS Running Buffer (Invitrogen, #NP002). .. Proteins were then transferred to a 0.45-μm nitrocellulose membrane (GE HealthCare, #10600002) and incubated overnight at 4°C with the appropriate primary antibodies: 1:50,000 β-actin (Proteintech, t#66009-1-Ig, RRID: AB_2687938), 1:1,000 G0S2 (Proteintech, cat. #12091-1-AP, RRID: AB_2877824), and 1:2,000 SRC (Cell Signaling Technology, #2110, RRID:AB_10691385) in Tris-buffered saline-Tween 20 (TBST; TBS tablets: EUROMEDEX, #EU1-7500-100; Tween 20: Sigma-Aldrich, #P2287) supplemented with 5% nonfat dry milk (Cell Signaling Technology, #999S) or 1:2,000 phospho-SRC Tyr 416 (Cell Signaling Technology, cat. #6943, RRID: AB_10013641) in TBST supplemented with 5% BSA (EUROMEDEX, # 04-100-812-C). .. On the next day, membranes were washed three times with TBST and incubated with the appropriate peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, #115-035-003).

    Saline:

    Article Title: An Extracellular Matrix–Producing Subset of Cancer-Associated Fibroblasts Drives Chemoresistance in Breast Cancer via SRC Activation and G0S2 Upregulation
    Article Snippet: Protein migration was performed for 1.5 hours at 150 V in 1× NuPAGE MES SDS Running Buffer (Invitrogen, #NP002). .. Proteins were then transferred to a 0.45-μm nitrocellulose membrane (GE HealthCare, #10600002) and incubated overnight at 4°C with the appropriate primary antibodies: 1:50,000 β-actin (Proteintech, t#66009-1-Ig, RRID: AB_2687938), 1:1,000 G0S2 (Proteintech, cat. #12091-1-AP, RRID: AB_2877824), and 1:2,000 SRC (Cell Signaling Technology, #2110, RRID:AB_10691385) in Tris-buffered saline-Tween 20 (TBST; TBS tablets: EUROMEDEX, #EU1-7500-100; Tween 20: Sigma-Aldrich, #P2287) supplemented with 5% nonfat dry milk (Cell Signaling Technology, #999S) or 1:2,000 phospho-SRC Tyr 416 (Cell Signaling Technology, cat. #6943, RRID: AB_10013641) in TBST supplemented with 5% BSA (EUROMEDEX, # 04-100-812-C). .. On the next day, membranes were washed three times with TBST and incubated with the appropriate peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, #115-035-003).



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    Regulation of myricetin on the activation of integrin α IIb β 3 and phosphorylation of integrin β 3 , Src, and Syk on a surface coated with fibrinogen. (A) Platelets (3.6 × 10 8 cells/mL) were either at (a) resting (Tyrode's solution) or preincubated with (b) 0.1% DMSO or myricetin (c, 10; d, 20 μM) and anti-PAC-1 mAb (2 μg/mL) for 3 min, after which they were stimulated with collagen (1 μg/mL) for 5 min. The number of fluorescein-labeled platelets was measured using a flow cytometer. In another experiments, washed platelets were preincubated with solvent control (0.1% DMSO) or myricetin (10 and 20 μM) and subsequently stimulated using immobilized fibrinogen (100 μg/mL). Subcellular extracts of the platelets were analyzed to determine the levels of phosphorylation of (B) integrin β 3 , (C) Src, and (D) Syk. Data are presented as the mean ± standard error of the mean ( n = 4). ** p < 0.01 and *** p < 0.001 compared with the resting (Tyrode's solution) group; # p < 0.05, ## p < 0.01, and ### p < 0.001 compared with the 0.1% DMSO + collagen group.

    Journal: Heliyon

    Article Title: Myricetin as a promising inhibitor of platelet fibrinogen receptor in humans

    doi: 10.1016/j.heliyon.2023.e20286

    Figure Lengend Snippet: Regulation of myricetin on the activation of integrin α IIb β 3 and phosphorylation of integrin β 3 , Src, and Syk on a surface coated with fibrinogen. (A) Platelets (3.6 × 10 8 cells/mL) were either at (a) resting (Tyrode's solution) or preincubated with (b) 0.1% DMSO or myricetin (c, 10; d, 20 μM) and anti-PAC-1 mAb (2 μg/mL) for 3 min, after which they were stimulated with collagen (1 μg/mL) for 5 min. The number of fluorescein-labeled platelets was measured using a flow cytometer. In another experiments, washed platelets were preincubated with solvent control (0.1% DMSO) or myricetin (10 and 20 μM) and subsequently stimulated using immobilized fibrinogen (100 μg/mL). Subcellular extracts of the platelets were analyzed to determine the levels of phosphorylation of (B) integrin β 3 , (C) Src, and (D) Syk. Data are presented as the mean ± standard error of the mean ( n = 4). ** p < 0.01 and *** p < 0.001 compared with the resting (Tyrode's solution) group; # p < 0.05, ## p < 0.01, and ### p < 0.001 compared with the 0.1% DMSO + collagen group.

    Article Snippet: Anti-phospho-p44/p42 extracellular signal–regulated kinase (ERK; Thr 202 /Tyr 204 ), anti-phospho- c -Jun N-terminal kinase (Thr 183 /Tyr 185 ), anti-phospho-phosphoinositide 3-kinase (PI3K) p85 (Tyr 458 )/p55 (Tyr 199 ), anti-phospho-(Ser) PKC substrate, phospho-Src family (Tyr 416 ; D49G4), and phospho-spleen tyrosine kinase (Syk; Tyr 525/526 ) pAbs were purchased from Cell Signaling (Beverly, MA, USA).

    Techniques: Activation Assay, Labeling, Flow Cytometry, Solvent